Activity of neutral and alkaline ceramidases on fluorogenic N-acylated coumarin-containing aminodiols
Ceramidases catalyze the cleavage of ceramides into sphingosine and fatty acids.Previously, we reported on the use of the RBM14 fluorogenic ceramide analogs to determine acidic ceramidase activity.In this work, we investigated the activity of other amidohydrolases on RBM14 compounds.Both bacterial and human purified neutral ceramidases (NCs), as we